Review





Similar Products

1h2 fab  (ATCC)
93
ATCC 1h2 fab
Figure 3. Neutralization and kinetics analysis of select VOCs and Fab fragments (A) Neutralization of SARS-CoV-2 pseudoviruses bearing the spike of the indicated VOC by the Fab of each indicated antibody. (B) IC50 values for each neutralization curve, calculated by nonlinear regression analysis. The L.O.D., shown as a dotted line, indicates the maximum concen- tration of antibody used in the experiment (5,000 or 50,000 ng/mL). For (A) and (B), error bars indicate the standard deviation from the mean of four experiments, each performed in technical duplicates. (C) SPR analysis of each Fab:spike pair, as indicated. The Fab ligand used for each experiment remains constant across each row, and the spike analyte remains constant for each column. The average KD obtained from eight replicate experiments is shown above each plot. Raw data are colored gray, with the 1:1 fit colored according to the spike concentration. For <t>1H2,</t> the dissociation was truncated to 450 s to capture an accurate off-rate. See also Tables S1 and S3.
1h2 Fab, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/1h2 fab/product/ATCC
Average 93 stars, based on 1 article reviews
1h2 fab - by Bioz Stars, 2026-02
93/100 stars
  Buy from Supplier
28772 1 ap  (Proteintech)
96
Proteintech 28772 1 ap
Figure 3. Neutralization and kinetics analysis of select VOCs and Fab fragments (A) Neutralization of SARS-CoV-2 pseudoviruses bearing the spike of the indicated VOC by the Fab of each indicated antibody. (B) IC50 values for each neutralization curve, calculated by nonlinear regression analysis. The L.O.D., shown as a dotted line, indicates the maximum concen- tration of antibody used in the experiment (5,000 or 50,000 ng/mL). For (A) and (B), error bars indicate the standard deviation from the mean of four experiments, each performed in technical duplicates. (C) SPR analysis of each Fab:spike pair, as indicated. The Fab ligand used for each experiment remains constant across each row, and the spike analyte remains constant for each column. The average KD obtained from eight replicate experiments is shown above each plot. Raw data are colored gray, with the 1:1 fit colored according to the spike concentration. For <t>1H2,</t> the dissociation was truncated to 450 s to capture an accurate off-rate. See also Tables S1 and S3.
28772 1 Ap, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/28772 1 ap/product/Proteintech
Average 96 stars, based on 1 article reviews
28772 1 ap - by Bioz Stars, 2026-02
96/100 stars
  Buy from Supplier
bromadiolone (cas: 28772-56-7, isomer ratio cis / trans : 72/18)  (Millipore)
90
Millipore bromadiolone (cas: 28772-56-7, isomer ratio cis / trans : 72/18)
Limits of detection (LOD) and quantification (LOQ) of warfarin (2), <t> bromadiolone </t> (12) and brodifacoum (15) achieved in food by different chromatographic techniques
Bromadiolone (Cas: 28772 56 7, Isomer Ratio Cis / Trans : 72/18), supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/bromadiolone (cas: 28772-56-7, isomer ratio cis / trans : 72/18)/product/Millipore
Average 90 stars, based on 1 article reviews
bromadiolone (cas: 28772-56-7, isomer ratio cis / trans : 72/18) - by Bioz Stars, 2026-02
90/100 stars
  Buy from Supplier

Image Search Results


Figure 3. Neutralization and kinetics analysis of select VOCs and Fab fragments (A) Neutralization of SARS-CoV-2 pseudoviruses bearing the spike of the indicated VOC by the Fab of each indicated antibody. (B) IC50 values for each neutralization curve, calculated by nonlinear regression analysis. The L.O.D., shown as a dotted line, indicates the maximum concen- tration of antibody used in the experiment (5,000 or 50,000 ng/mL). For (A) and (B), error bars indicate the standard deviation from the mean of four experiments, each performed in technical duplicates. (C) SPR analysis of each Fab:spike pair, as indicated. The Fab ligand used for each experiment remains constant across each row, and the spike analyte remains constant for each column. The average KD obtained from eight replicate experiments is shown above each plot. Raw data are colored gray, with the 1:1 fit colored according to the spike concentration. For 1H2, the dissociation was truncated to 450 s to capture an accurate off-rate. See also Tables S1 and S3.

Journal: Cell reports

Article Title: Potent Omicron-neutralizing antibodies isolated from a patient vaccinated 6 months before Omicron emergence.

doi: 10.1016/j.celrep.2023.112421

Figure Lengend Snippet: Figure 3. Neutralization and kinetics analysis of select VOCs and Fab fragments (A) Neutralization of SARS-CoV-2 pseudoviruses bearing the spike of the indicated VOC by the Fab of each indicated antibody. (B) IC50 values for each neutralization curve, calculated by nonlinear regression analysis. The L.O.D., shown as a dotted line, indicates the maximum concen- tration of antibody used in the experiment (5,000 or 50,000 ng/mL). For (A) and (B), error bars indicate the standard deviation from the mean of four experiments, each performed in technical duplicates. (C) SPR analysis of each Fab:spike pair, as indicated. The Fab ligand used for each experiment remains constant across each row, and the spike analyte remains constant for each column. The average KD obtained from eight replicate experiments is shown above each plot. Raw data are colored gray, with the 1:1 fit colored according to the spike concentration. For 1H2, the dissociation was truncated to 450 s to capture an accurate off-rate. See also Tables S1 and S3.

Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Negative stain EM map of SARS-CoV-2 D614G Spike in complex with 2G3 IgG This study EMD-28770 Negative stain EM map of SARS-CoV-2 D614G Spike in complex with 2E6 IgG This study EMD-28771 Negative stain EM map of SARS-CoV-2 D614G Spike in complex with 1H2 Fab This study EMD-28772 Negative stain EM map of SARS-CoV-2 D614G Spike in complex with 1G8 IgG This study EMD-28773 Experimental models: Cell lines 293T cells ATCC Cat# CRL-3216 Vero cells ATCC Cat# CRL-1586 ExpiCho-S cells Thermo Fisher Scientific Cat# A29127 Recombinant DNA Empty vector: phCMV3 Genlantis Cat# P003300 pCAGGS-VSV-G Kerfast Cat# EH1017 phCMV3-D614G Spike This study GenBank #QHD43416.1 with D614G mutation phCMV3-Beta Spike This study GenBank #QHD43416.1 with L18F, D80A, D215G, D242–244, R246I, K417N, E484K, N501Y, D614G, and A701V mutations phCMV3-Delta Spike This study GenBank #QHD43416.1 with T19R, G142D, E156G, D157–158, L452R, T478K, D614G, P681R, and D950N mutations phCMV3-Omicron BA.1 Spike This study GenBank #QHD43416.1 with A67V, D69/70, T95I, G142D, D143/145, N211I, D212, ins214 EPE, G339D, S371L, S373P, S375F, S477N, T478K, E484A, Q493R, G496S, Q498R, N501Y, Y505H, T547K, D614G, H655Y, N679K, P681H, D796Y, N856K, Q954H, N969K, and L981F mutations phCMV3-Omicron BA1.1 Spike This study GenBank #QHD43416.1 with A67V, D69/70, T95I, G142D, D143/145, N211I, D212, ins214 EPE, G339D, R346K, S371L, S373P, S375F, S477N, T478K, E484A, Q493R, G496S, Q498R, N501Y, Y505H, T547K, D614G, H655Y, N679K, P681H, D796Y, N856K, Q954H, N969K, and L981F mutations phCMV3-Omicron BA.2 Spike This study GenBank #QHD43416.1 with T19I, L24S, D25/27, G142D, V213G, ins214 EPE, G339D, S371F, S373P, S375F, T376A, D405N, R408S, K417N, N440K, S477N, T478K, E484A, Q493R, Q498R, N501Y, Y505H, D614G, H655Y, N679K, P681H, N764K, D796Y, Q954H, and N969K mutations phCMV3-Omicron BA.2.12.1 Spike This study GenBank #QHD43416.1 with T19I, L24S, D25/27, G142D, V213G, ins214 EPE, G339D, S371F, S373P, S375F, T376A, D405N, R408S, K417N, N440K, L452Q, S477N, T478K, E484A, Q493R, Q498R, N501Y, Y505H, D614G, H655Y, N679K, P681H, S740L, N764K, D796Y, Q954H, and N969K mutations (Continued on next page) Cell Reports 42, 112421, May 30, 2023 19

Techniques: Neutralization, Standard Deviation, Concentration Assay

Limits of detection (LOD) and quantification (LOQ) of warfarin (2), bromadiolone (12) and brodifacoum (15) achieved in food by different chromatographic techniques

Journal: Analytical and Bioanalytical Chemistry

Article Title: Methods in food defence: a simple and robust screening for 16 rodenticides by LC-DAD/FLD following QuEChERS–based extraction

doi: 10.1007/s00216-022-04145-w

Figure Lengend Snippet: Limits of detection (LOD) and quantification (LOQ) of warfarin (2), bromadiolone (12) and brodifacoum (15) achieved in food by different chromatographic techniques

Article Snippet: Brodifacoum (CAS: 56073-10-0, isomer ratio cis / trans : 58/41), bromadiolone (CAS: 28772-56-7, isomer ratio cis / trans : 72/18), coumatetralyl (CAS: 5836-29-3), difenacoum (CAS: 56073-07-5, isomer ratio cis / trans : 53/46), flocoumafen (CAS: 90035-08-8, isomer ratio cis / trans : 21/79) and warfarin (CAS: 81-81-2, racemate) were from Sigma-Aldrich (Schnelldorf, Germany).

Techniques:

LC-DAD/FLD chromatograms (DAD: 310 nm, FLD: excitation 310 nm, emission 390 nm) showing baseline separation of all of the rodenticides (coumafuryl ( 1 ), warfarin ( 2 ), phenprocoumon ( 3 ), acenocoumarol ( 4 ), coumatetralyl ( 5 ), coumachlor ( 6 ), diphacinone ( 7 ), dicoumarol ( 8 ), tioclomarol ( cis : 9a , trans : 9b ), chlorophacinone ( 10 ), ferulenol ( 11 ), bromadiolone ( cis : 12a , trans : 12b ), difenacoum ( 13 ), flocoumafen ( cis : 14a , trans : 14b ), brodifacoum ( 15 ), difethialone ( 16 ); 0.01 µg/mL: 5 ; 0.1 µg/mL: 1–3 , 6 , 9a/b , 11–15 ; 1 µg/mL: 4 , 7 , 8 , 10 , 16 ) by ion-pair chromatography using a biphenyl column (150 mm × 4.6 mm, 2.6 µm) and gradient elution (solvent A: 10 mmol/L acetic acid/DIPEA in water, solvent B: 10 mmol/L acetic acid/DIPEA in methanol, flow rate: 0.7 mL/min, min/% B: 0/48, 10/78, 25/98, 30/48, 40 °C)

Journal: Analytical and Bioanalytical Chemistry

Article Title: Methods in food defence: a simple and robust screening for 16 rodenticides by LC-DAD/FLD following QuEChERS–based extraction

doi: 10.1007/s00216-022-04145-w

Figure Lengend Snippet: LC-DAD/FLD chromatograms (DAD: 310 nm, FLD: excitation 310 nm, emission 390 nm) showing baseline separation of all of the rodenticides (coumafuryl ( 1 ), warfarin ( 2 ), phenprocoumon ( 3 ), acenocoumarol ( 4 ), coumatetralyl ( 5 ), coumachlor ( 6 ), diphacinone ( 7 ), dicoumarol ( 8 ), tioclomarol ( cis : 9a , trans : 9b ), chlorophacinone ( 10 ), ferulenol ( 11 ), bromadiolone ( cis : 12a , trans : 12b ), difenacoum ( 13 ), flocoumafen ( cis : 14a , trans : 14b ), brodifacoum ( 15 ), difethialone ( 16 ); 0.01 µg/mL: 5 ; 0.1 µg/mL: 1–3 , 6 , 9a/b , 11–15 ; 1 µg/mL: 4 , 7 , 8 , 10 , 16 ) by ion-pair chromatography using a biphenyl column (150 mm × 4.6 mm, 2.6 µm) and gradient elution (solvent A: 10 mmol/L acetic acid/DIPEA in water, solvent B: 10 mmol/L acetic acid/DIPEA in methanol, flow rate: 0.7 mL/min, min/% B: 0/48, 10/78, 25/98, 30/48, 40 °C)

Article Snippet: Brodifacoum (CAS: 56073-10-0, isomer ratio cis / trans : 58/41), bromadiolone (CAS: 28772-56-7, isomer ratio cis / trans : 72/18), coumatetralyl (CAS: 5836-29-3), difenacoum (CAS: 56073-07-5, isomer ratio cis / trans : 53/46), flocoumafen (CAS: 90035-08-8, isomer ratio cis / trans : 21/79) and warfarin (CAS: 81-81-2, racemate) were from Sigma-Aldrich (Schnelldorf, Germany).

Techniques: Chromatography

Linear working ranges observed in eluent as well as limits of detection (LOD) and quantification (LOQ) obtained from blank matrix analysis following the developed protocol (wheat flour, n = 10)

Journal: Analytical and Bioanalytical Chemistry

Article Title: Methods in food defence: a simple and robust screening for 16 rodenticides by LC-DAD/FLD following QuEChERS–based extraction

doi: 10.1007/s00216-022-04145-w

Figure Lengend Snippet: Linear working ranges observed in eluent as well as limits of detection (LOD) and quantification (LOQ) obtained from blank matrix analysis following the developed protocol (wheat flour, n = 10)

Article Snippet: Brodifacoum (CAS: 56073-10-0, isomer ratio cis / trans : 58/41), bromadiolone (CAS: 28772-56-7, isomer ratio cis / trans : 72/18), coumatetralyl (CAS: 5836-29-3), difenacoum (CAS: 56073-07-5, isomer ratio cis / trans : 53/46), flocoumafen (CAS: 90035-08-8, isomer ratio cis / trans : 21/79) and warfarin (CAS: 81-81-2, racemate) were from Sigma-Aldrich (Schnelldorf, Germany).

Techniques:

Bias (mean recovery, %) and repeatability (relative standard deviation, %) rates obtained from blank matrix samples spiked to the determined LOQ of each rodenticide. Experiments were carried out with and without applying an additional solvent exchange to evaluate sufficient chromatographic stability when omitting this time-consuming, additional step ( n = 8)

Journal: Analytical and Bioanalytical Chemistry

Article Title: Methods in food defence: a simple and robust screening for 16 rodenticides by LC-DAD/FLD following QuEChERS–based extraction

doi: 10.1007/s00216-022-04145-w

Figure Lengend Snippet: Bias (mean recovery, %) and repeatability (relative standard deviation, %) rates obtained from blank matrix samples spiked to the determined LOQ of each rodenticide. Experiments were carried out with and without applying an additional solvent exchange to evaluate sufficient chromatographic stability when omitting this time-consuming, additional step ( n = 8)

Article Snippet: Brodifacoum (CAS: 56073-10-0, isomer ratio cis / trans : 58/41), bromadiolone (CAS: 28772-56-7, isomer ratio cis / trans : 72/18), coumatetralyl (CAS: 5836-29-3), difenacoum (CAS: 56073-07-5, isomer ratio cis / trans : 53/46), flocoumafen (CAS: 90035-08-8, isomer ratio cis / trans : 21/79) and warfarin (CAS: 81-81-2, racemate) were from Sigma-Aldrich (Schnelldorf, Germany).

Techniques: Standard Deviation